rabbit 114 anti total s6k  (Cell Signaling Technology Inc)

Journal: Molecular therapy. Nucleic acids

Article Title: DNM2 levels normalization improves muscle phenotypes of a novel mouse model for moderate centronuclear myopathy.

doi: 10.1016/j.omtn.2023.07.003

Figure Lengend Snippet: Figure 4. Muscle structure disorganization in Dnm2R369W/+ mice (A) Different populations of mitochondria and triads found in electron microscopy: normal mitochondria (NM), enlarged mitochondria (EM), damaged mitochondria (DM, with abnormal cristae), normal triad (NT) and altered T-tubule (AT) (n = 2). Bars in the larger panel and mitochondria represent 1 mm; bars in the triads represent 0.1 mm. (B) Prohibitin protein levels (30 kDa, n = 3), (C) mRNA level of Sirt1 and protein levels of (D) PARK2 (55 kDa, n = 4), and (E) phosphorylated and total S6K (60 kDa, n = 5). (F) BIN1 and DHPR localization in longitudinal muscle sections (n = 3, bar represents 10 mm) and (G) BIN1 protein expression (n = 5, 60 kDa). (H) Desmin mislocalization (arrows) in transverse muscle sections (n = 3). Mice at 8 weeks old. Bar represents 50 mm. Mean ± SEM. Unpaired t test, *p < 0.05 and **p < 0.01: Dnm2R369W/+ differs from WT.

Article Snippet: The membranes were blocked in 5% milk and incubated with anti-BIN1 (1:20,000, homemade 2405), anti-DNM2 (1:50,000, homemade 2865), anti-GAPDH (1:10,000, Millipore MAB374, Burlington, MA, USA), anti-OXPHOS (1:500, ABCAM ab110413, Cambridge, United Kingdom), anti-PARK2 (1:3,000, Invitrogen, 702785, Waltham, MA, USA), anti-prohibitin (1:1000, ABCAM, ab28172, Cambridge, United Kingdom), anti-PYGM (1:1000, ABCAM ab63158, Cambridge, United Kingdom), anti-total S6K (1:1000, Cell Signaling, 2708, Danvers, MA, USA) or anti-phosphorylated S6K (1:1000, Cell Signaling, 9208, Danvers, MA, USA) and for 1 h with 1:10,000 secondary antibodies conjugated with horseradish.

Techniques: Electron Microscopy, Expressing

Journal: iScience

Article Title: A novel method for visualizing in-vivo rates of protein degradation provides insight into how TRIM28 regulates muscle size

doi: 10.1016/j.isci.2023.106526

Figure Lengend Snippet: Primary

Article Snippet: Rabbit Anti-Total P70 S6K , Cell Signaling Technologies (Danvers, MA, USA) , Cat# 2708S; RRID:AB_390722.

Techniques: Plasmid Preparation, Marker, Injection, DC Protein Assay, SYBR Green Assay, Mutagenesis, Sample Prep, Software